- Product Name :
- Sumo1, Human
( Small ubiquitin-related modifier)
- Storage :
- Shipped at 4°C. Store at -20°C. After reconstitution, store at -80°C and avoid frequent freeze and thaw.
- Composition :
- Lyophilized from 20mM Tris, pH 8.0 / 10mM NaCl
- Source :
- Purified from E.coli expressing the human SUMO1 gene (1-97aa, with additional 3 amino acids-GSH at N-term)
- Molecular Weight :
- 11.7 kDa
- Endotoxin activity :
- Not tested
- Purity :
- > 95% by SDS-PAGE
- Expression system :
- Background :
- The small ubiquitin-related modifier (SUMO) belongs to the growing family of ubiquitin-related proteins involved in posttranslational protein modification. It is present in all eukaryotic kingdoms and is highly conserved from yeast to humans. The SUMO family are composed of three types; SUMO1, SUMO2 and SUMO3. Like ubiquitin, SUMO1 is conjugated to target proteins by an enzymatic cascade involving E1, E2 enzyme and E3 lygase (known as sumoylation). It has been known that many substrates such as RanGAP1, PML and IkBa proteins are present in cells. In contrast to ubiquitination, sumoylation does not tag proteins for degradation by the 26S proteosome. Sumoylation participates in diverse events such as nuclear transport, transcriptional regulation, chromosome segregation and cell cycle control. Also the modification is associated with pathological conditions (neurodegeneration, diabetes and Inflammation).
- Background reference :
- 1) Muller S. et al., 2001, Nat Rev Mol Cell Biol. 2:202-210.
2) Gill G, 2004, Genes Dev. 18(17):2046-2059.
3) Hay RT, 2005, Mol Cell. 18(1):1-12.
4) Degerny C et al., 2005, J Biol Chem. 280(26):24330-24338.
- Database link - SwissProt no.
The test was performed by customers with their own sample.