- Product Name :
- Thioredoxin Reductase, Yeast
- Storage :
- Shipped at 4°C. Store at -20°C. After reconstitution, store at -80°C and avoid frequent freeze and thaw.
- Composition :
- Lyophilized from 20mM HEPES, pH 7.5, 1mM EDTA
- Source :
- Purified from E.coli expressing the yeast Thioredoxin Reductase gene (1-319aa)
- Molecular Weight :
- 34.3 kDa
- Specific activity :
- 6U/mg(One unit will cause the oxidation of 1.0 μmole of NADPH per minute by ΔA412/0.0136×2)
- Endotoxin activity :
- Not tested
- Purity :
- > 95% by SDS-PAGE
- Expression system :
- E.coli
- Background :
- Yeast thioredoxin reductase is a flavoprotein with a molecular weight of approx. 35,000 and consist of two subunits, each containing one molecule of FAD and catayzes the NADPH-dependent reduction of thioredoxin.
- Background reference :
- 1) Minchiotti, et al. (1973) Biochemica et Biophysica Acta.327.274-281.
- Research area :
-
ROS Signaling
The test was performed by customers with their own sample.